A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species.
Identifieur interne : 001081 ( Main/Exploration ); précédent : 001080; suivant : 001082A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species.
Auteurs : Carla Freitas Munhoz [Brésil] ; Zirlane Portugal Costa [Brésil] ; Luiz Augusto Cauz-Santos [Brésil] ; Alina Carmen Egoávil Reátegui [Brésil] ; Nathalie Rodde [France] ; Stéphane Cauet [France] ; Marcelo Carnier Dornelas [Brésil] ; Philippe Leroy [France] ; Alessandro De Mello Varani [Brésil] ; Hélène Bergès [France] ; Maria Lucia Carneiro Vieira [Brésil]Source :
- Scientific reports [ 2045-2322 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Manihot, Passiflora, Populus.
- Analyse de séquence d'ADN, Banque de gènes, Génome végétal, Ordre des gènes, Synténie.
English descriptors
- KwdEn :
- MESH :
- genetics : Manihot, Passiflora, Populus.
- Gene Library, Gene Order, Genome, Plant, Sequence Analysis, DNA, Synteny.
Abstract
Passiflora edulis is the most widely cultivated species of passionflowers, cropped mainly for industrialized juice production and fresh fruit consumption. Despite its commercial importance, little is known about the genome structure of P. edulis. To fill in this gap in our knowledge, a genomic library was built, and now completely sequenced over 100 large-inserts. Sequencing data were assembled from long sequence reads, and structural sequence annotation resulted in the prediction of about 1,900 genes, providing data for subsequent functional analysis. The richness of repetitive elements was also evaluated. Microsyntenic regions of P. edulis common to Populus trichocarpa and Manihot esculenta, two related Malpighiales species with available fully sequenced genomes were examined. Overall, gene order was well conserved, with some disruptions of collinearity identified as rearrangements, such as inversion and translocation events. The microsynteny level observed between the P. edulis sequences and the compared genomes is surprising, given the long divergence time that separates them from the common ancestor. P. edulis gene-rich segments are more compact than those of the other two species, even though its genome is much larger. This study provides a first accurate gene set for P. edulis, opening the way for new studies on the evolutionary issues in Malpighiales genomes.
DOI: 10.1038/s41598-018-31330-8
PubMed: 30158558
PubMed Central: PMC6115403
Affiliations:
- Brésil, France
- Auvergne (région administrative), Auvergne-Rhône-Alpes, Midi-Pyrénées, Occitanie (région administrative), État de São Paulo
- Campinas, Castanet-Tolosan, Clermont-Ferrand, São Paulo
- Université d'État de Campinas, Université de São Paulo
Links toward previous steps (curation, corpus...)
Le document en format XML
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Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil. mlcvieir@usp.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30158558</idno><idno type="pmid">30158558</idno><idno type="doi">10.1038/s41598-018-31330-8</idno><idno type="pmc">PMC6115403</idno><idno type="wicri:Area/Main/Corpus">000C85</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C85</idno><idno type="wicri:Area/Main/Curation">000C85</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C85</idno><idno type="wicri:Area/Main/Exploration">000C85</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species.</title><author><name sortKey="Munhoz, Carla Freitas" sort="Munhoz, Carla Freitas" uniqKey="Munhoz C" first="Carla Freitas" last="Munhoz">Carla Freitas Munhoz</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Costa, Zirlane Portugal" sort="Costa, Zirlane Portugal" uniqKey="Costa Z" first="Zirlane Portugal" last="Costa">Zirlane Portugal Costa</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Cauz Santos, Luiz Augusto" sort="Cauz Santos, Luiz Augusto" uniqKey="Cauz Santos L" first="Luiz Augusto" last="Cauz-Santos">Luiz Augusto Cauz-Santos</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Reategui, Alina Carmen Egoavil" sort="Reategui, Alina Carmen Egoavil" uniqKey="Reategui A" first="Alina Carmen Egoávil" last="Reátegui">Alina Carmen Egoávil Reátegui</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Rodde, Nathalie" sort="Rodde, Nathalie" uniqKey="Rodde N" first="Nathalie" last="Rodde">Nathalie Rodde</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet-Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Cauet, Stephane" sort="Cauet, Stephane" uniqKey="Cauet S" first="Stéphane" last="Cauet">Stéphane Cauet</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet-Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Dornelas, Marcelo Carnier" sort="Dornelas, Marcelo Carnier" uniqKey="Dornelas M" first="Marcelo Carnier" last="Dornelas">Marcelo Carnier Dornelas</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Campinas, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Campinas</wicri:regionArea><orgName type="university">Université d'État de Campinas</orgName><placeName><settlement type="city">Campinas</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author><author><name sortKey="Leroy, Philippe" sort="Leroy, Philippe" uniqKey="Leroy P" first="Philippe" last="Leroy">Philippe Leroy</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UCA, UMR 1095, GDEC, 63000, Clermont-Ferrand, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UCA, UMR 1095, GDEC, 63000, Clermont-Ferrand</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Clermont-Ferrand</settlement></placeName></affiliation></author><author><name sortKey="Varani, Alessandro De Mello" sort="Varani, Alessandro De Mello" uniqKey="Varani A" first="Alessandro De Mello" last="Varani">Alessandro De Mello Varani</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900, Jaboticabal, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900, Jaboticabal</wicri:regionArea><wicri:noRegion>Jaboticabal</wicri:noRegion></affiliation></author><author><name sortKey="Berges, Helene" sort="Berges, Helene" uniqKey="Berges H" first="Hélène" last="Bergès">Hélène Bergès</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan</wicri:regionArea><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Midi-Pyrénées</region><settlement type="city">Castanet-Tolosan</settlement></placeName></affiliation></author><author><name sortKey="Vieira, Maria Lucia Carneiro" sort="Vieira, Maria Lucia Carneiro" uniqKey="Vieira M" first="Maria Lucia Carneiro" last="Vieira">Maria Lucia Carneiro Vieira</name><affiliation wicri:level="4"><nlm:affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil. mlcvieir@usp.br.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba</wicri:regionArea><orgName type="university">Université de São Paulo</orgName><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName></affiliation></author></analytic><series><title level="j">Scientific reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Library (MeSH)</term><term>Gene Order (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Manihot (genetics)</term><term>Passiflora (genetics)</term><term>Populus (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Synteny (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Génome végétal (MeSH)</term><term>Manihot (génétique)</term><term>Ordre des gènes (MeSH)</term><term>Passiflora (génétique)</term><term>Populus (génétique)</term><term>Synténie (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Manihot</term><term>Passiflora</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Manihot</term><term>Passiflora</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Library</term><term>Gene Order</term><term>Genome, Plant</term><term>Sequence Analysis, DNA</term><term>Synteny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Banque de gènes</term><term>Génome végétal</term><term>Ordre des gènes</term><term>Synténie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Passiflora edulis is the most widely cultivated species of passionflowers, cropped mainly for industrialized juice production and fresh fruit consumption. Despite its commercial importance, little is known about the genome structure of P. edulis. To fill in this gap in our knowledge, a genomic library was built, and now completely sequenced over 100 large-inserts. Sequencing data were assembled from long sequence reads, and structural sequence annotation resulted in the prediction of about 1,900 genes, providing data for subsequent functional analysis. The richness of repetitive elements was also evaluated. Microsyntenic regions of P. edulis common to Populus trichocarpa and Manihot esculenta, two related Malpighiales species with available fully sequenced genomes were examined. Overall, gene order was well conserved, with some disruptions of collinearity identified as rearrangements, such as inversion and translocation events. The microsynteny level observed between the P. edulis sequences and the compared genomes is surprising, given the long divergence time that separates them from the common ancestor. P. edulis gene-rich segments are more compact than those of the other two species, even though its genome is much larger. This study provides a first accurate gene set for P. edulis, opening the way for new studies on the evolutionary issues in Malpighiales genomes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30158558</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>04</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>08</Month><Day>29</Day></PubDate></JournalIssue><Title>Scientific reports</Title><ISOAbbreviation>Sci Rep</ISOAbbreviation></Journal><ArticleTitle>A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species.</ArticleTitle><Pagination><MedlinePgn>13024</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41598-018-31330-8</ELocationID><Abstract><AbstractText>Passiflora edulis is the most widely cultivated species of passionflowers, cropped mainly for industrialized juice production and fresh fruit consumption. Despite its commercial importance, little is known about the genome structure of P. edulis. To fill in this gap in our knowledge, a genomic library was built, and now completely sequenced over 100 large-inserts. Sequencing data were assembled from long sequence reads, and structural sequence annotation resulted in the prediction of about 1,900 genes, providing data for subsequent functional analysis. The richness of repetitive elements was also evaluated. Microsyntenic regions of P. edulis common to Populus trichocarpa and Manihot esculenta, two related Malpighiales species with available fully sequenced genomes were examined. Overall, gene order was well conserved, with some disruptions of collinearity identified as rearrangements, such as inversion and translocation events. The microsynteny level observed between the P. edulis sequences and the compared genomes is surprising, given the long divergence time that separates them from the common ancestor. P. edulis gene-rich segments are more compact than those of the other two species, even though its genome is much larger. This study provides a first accurate gene set for P. edulis, opening the way for new studies on the evolutionary issues in Malpighiales genomes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Munhoz</LastName><ForeName>Carla Freitas</ForeName><Initials>CF</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Costa</LastName><ForeName>Zirlane Portugal</ForeName><Initials>ZP</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cauz-Santos</LastName><ForeName>Luiz Augusto</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reátegui</LastName><ForeName>Alina Carmen Egoávil</ForeName><Initials>ACE</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rodde</LastName><ForeName>Nathalie</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cauet</LastName><ForeName>Stéphane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dornelas</LastName><ForeName>Marcelo Carnier</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Campinas, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leroy</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>INRA, UCA, UMR 1095, GDEC, 63000, Clermont-Ferrand, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Varani</LastName><ForeName>Alessandro de Mello</ForeName><Initials>AM</Initials><Identifier Source="ORCID">0000-0002-8876-3269</Identifier><AffiliationInfo><Affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900, Jaboticabal, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bergès</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique (INRA), Centre National de Ressources Génomique Végétales, 31326, Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vieira</LastName><ForeName>Maria Lucia Carneiro</ForeName><Initials>MLC</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, 13418-900, Piracicaba, Brazil. mlcvieir@usp.br.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>08</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Sci Rep</MedlineTA><NlmUniqueID>101563288</NlmUniqueID><ISSNLinking>2045-2322</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D023061" MajorTopicYN="Y">Gene Order</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="Y">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002365" MajorTopicYN="N">Manihot</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029598" MajorTopicYN="N">Passiflora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D026801" MajorTopicYN="Y">Synteny</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>8</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>8</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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